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Data showing increased sensitivity
Data courtesy of: Harvard Medical School, Partners
Healthcare System
Cambridge, MA |
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| Coverslip
Hybridization @ 42° C, 16hrs. |
MAUI
Hybridization @ 42° C, 16hrs. |
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Experimental results
1. Average spot size detection increase = 15%
2. Average raw signal Cy5 increase = >5X
3. Average raw signal Cy3 increase = >5X
4. Average background = same
5. Spot detection increase = 2.3X (from 32% to 73%)
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Data
showing reduced hybridization times
Data courtesy of: DNA Chip Research, Inc., JAPAN. |
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Coverslip Hybridization
16hrs./Cy3/Scanner PMT65 |
MAUI® Hybridization System
4hrs./Cy3/Scanner PMT65 |
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Data showing increased
sensitivity due to mixing
Data Courtesy of: The Analytical Chemistry
article, Gains in sensitivity with a device that mixes microarray
hybridization solution in a 25-um-thick chamber. Anal.
Chem. 74: 6413-6417.
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Mixing of the hybridization solution
significantly improves sensitivity from low abundance
labeled targets. The following quantity of labeled
bacteriophage lambda PCR product targets were used (fMol/slide):
A = 22, B = 1.1, C = 0.10, D = 0.0094. Every microarray
spot contained approximately 0.1 fMol (100 aMol) probe.
Error bar represents one standard deviation of 22 identical
microarray spots. |
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